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Test ID: ebv-EArly™

ebv-EArly™ is based on the detection of IgA antibodies to Epstein-Barr Virus (EBV) Early Antigen (Ea) using indirect immunofluorescence. This assay is the gold standard recommended by MOH* for detecting NPC in high risk individuals. We recommend this test for most patients.

*MOH Clinical Practice Guidelines 1/2010. Ministry of Health, Singapore.

Test ID: ebv-2stack™

ebv-2stack™ is the stacking of ebv-EArly™ with an additional assay, the detection of IgA antibodies to EBV Viral Capsid Protein (VCA). We recommend this test only for patients who have established a baseline for VCA and wish to continue monitoring it.

Useful for

Detection of EBV reactivation which is a strong indicator of the presence of nasopharyngeal carcinoma (NPC)

Clinical Information

Using indirect immunofluorescent staining techniques, specific cellular staining patterns observed in NPC-positive patients are measured in these assays.

Reference Values

Antibody titre: Negative, 1:5, 1:10, 1:40, 1:160, 1:640

Interpretation: Antibody titres >1:5 are positive and the titre magnitude is correlated with disease burden and prognosis.


  • Turnaround time: 2-4 days
  • Test Days: Mondays & Thursdays
  • Download and fill 'Test Request Form' and send with blood sample(s).

Courier service

  • Call or WhatsApp us at 90-39-39-90 for sample collection
  • Minimum 2cc of blood (preferably in a 5 mL vacutainer)
  • Storage (same day): Room Temp (preferably 4°C)
  • Storage (overnight): 4°C


Epstein Barr virus (EBV) antibodies in the diagnosis of NPC--comparison between IFA and two commercial ELISA kits. Chan SH, Soo MY, Gan YY, Fones-Tan A, Sim PS, Kaur A, Chew CT. Singapore Med J. 1998 Jun;39(6):263-5.

Increasing the accuracy and scalability of the Immunofluorescence Assay for Epstein Barr Virus by inferring continuous titers from a single sample dilution. Goh SM, Swaminathan M, Lai JU, Anwar A, Chan SH, Cheong I. J Immunol Methods. 2017 Jan;440:35-40.

Chan, Soh Ha, Christopher Hood Keng Goh, Joseph Tien Seng Wee, Cam Ha Tam, Luke Kim Siang Tan, Derrick Heng, and Mun Tuck Lee. 2010. MOH Clinical Practice Guidelines 1/2010. Ministry of Health, Singapore.

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Disclaimer : The information contained in this website is provided for informational purposes only, and should not be construed as medical advice on any matter. Please contact us for specific queries.​